Semicoordinated allelic-bursting shape dynamic random monoallelic expression in pregastrulation embryos

Summary Recently, allele-specific single-cell RNA-seq analysis has demonstrated widespread dynamic random monoallelic expression of autosomal genes (aRME) in different cell types. However, the prevalence aRME during pregastrulation remains unknown. Here, we show that is lineages embryos. Additionally, origin elusive. It believed independent transcriptional bursting from each allele leads to aRME. allelic burst not perfectly independent; instead it happens a semicoordinated fashion. Importantly, genes, particularly with low frequency, frequent asynchronous bursting, thereby contributing Furthermore, found coordination lineage specific and regulating development have higher degree coordination. Altogether, our study provides significant insights into their developmental relevance early development.